Document Details

Document Type : Article In Journal 
Document Title :
mono-Brominated-Propyphenazone as a New Derivatization Reagent for High Performance Liquid Chromatography of Ephedrine with UV-Detection
ببروموميثيل-بروبيفينازون كعامل اشتقاق جديد لتحليل الإافدرين بكروماتوجرافيا السوائل ذات الأداء العالي مع الكشف بالأشعة فوق البنفسجية
 
Subject : Original Articles 
Document Language : English 
Abstract : mono-brominated-propyphenazone was used as a derivatization reagent for the detection and quantification of ephedrine by high performance liquid chromatography using Zorbax-XDB C18 column, and 0.02 M sodium acetate (pH 4.4): acetonitrile (52:48 v/v) as mobile phase and UV detection at 280nm. The cited reagent reacts with the secondary amino group of ephedrine in acetone using anhydrous potassium carbonate as hydrobromide acceptor. The reaction was completed within 20 minutes in 100 ?L acetone with heating at 90 ± 5°C in a capped mini reaction vial. The stoichiometry of the reaction was one-to-one mol from each mono-brominated-propyphenazone and ephedrine. The linear concentration range of ephedrine was 30 to 900 ng per injection (50 µL). The detection limit was 9 ng/injection (50 µL). The derivatized ephedrine was synthesized and confirmed with spectral analysis. This method was applied for determination of spiked ephedrine in human urine and after extraction with charcoal column using methanol: 0.2 M NaOH (20:0.2, mL:mL) as eluant. 
ISSN : 1319-1004 
Journal Name : Medical Science Journal 
Volume : 15 
Issue Number : 1 
Publishing Year : 1429 AH
2008 AD 
Number Of Pages : 14 
Article Type : Article 
Added Date : Sunday, October 11, 2009 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
علاءالدين محمود خضر Alaa E. M. Khedr Researcher  
فايدة حسن بامانعFayda H. BamaneResearcher  

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