Document Details
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Document Type |
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Article In Conference |
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Document Title |
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Neonatal Screening for Hemoglobinopathies Using High-Performance Liquid Chromatography استخدام جهاز قياس انسيابية الوسائل عالي الأداء (HPLC) في اكتشاف حالات اعتلالات خاضاب الدم الوراثي لدى المواليد |
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Subject |
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medicine, dentistry and pharmacological sciences |
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Document Language |
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Arabic |
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Abstract |
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Methods of screening for hemoglobin variants in neonatal samples are similar
to those used for adults, but are subject to several constraints. Neonatal blood consists
of ~75% fetal hemoglobin (HbF). Any method used must therefore be sufficiently
sensitive to detect low levels of normal and abnormal hemoglobins, as well as the
absence of very small quantities of normal adult hemoglobin (HbA).
The primary aim of neonatal screening is therefore to identify infants with
major significant hemoglobinopathies disorders,· which include sickle cell anemia
(HbFS), HbFSC disease, HbFSD, HbSO Arab, HbS/beta. thalassemia and beta
thalassemia major. These disorders identify by the absence of HbA and the presence
of abnormal variant Hb [1, 3. 12,21]. A secondary outcome is that they,will also
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detect individuals with a variety of moderate and minor hemoglobin disorders, such as
alpha thalassemia (a-thalassemia), heterozygotes for a Hb variant, or carriers of a
clinically benign variant e.g. sickle cell trait (HbFAS).
Another outcome is the identification of the babys own genetic risk, which at
this stage can only be communicated to the parents and included in the primary
healthcare record.
The conventional approach to qualitative and quantitative analyses of
hemoglobin (Hb) molecules for diagnosis of hemoglobinopathies requires a
combination of tests. The preferred laboratory method in most hospitals using twintier
electrophoresis using citrate agar and cellulose acetate, with the second most
common method being isoelectric focusing. The lack of a quantitative aspect to the
isoelectric focusing methods and other technical limitations relating to lack of
sensitivity or specificity for the twin-tiered method [1-4]. High-Performance Liquid
Chromatography (HPLC) coupled with the development of an automated and rapid
method [5], HPLC achieves excellent sensitivity and specificity, while adding the
very important quantitative element to the analysis, [6-15], also has proven to be
clinically accurate [16, 17].
Reviewing the literature it has become apparent ,that HPLC is the preferred
method for screening that lead us to use an automated HPLC (VARIANT) system
from BIO-RAD, to study alph-thalassemia and other hemoglobinopathies in our
Newborn Screen In Jeddah. |
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Conference Name |
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the second annual meeting for scientific research |
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Duration |
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From : 27 محرم AH - To : 28 محرم AH
From : 30 مارس AD - To : 31 مارس AD |
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Publishing Year |
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1424 AH
2003 AD |
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Number Of Pages |
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12 |
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Article Type |
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Article |
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Added Date |
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Tuesday, January 13, 2009 |
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Researchers
| سعاد الجوعاني | AI-Jaouni, N/A | Investigator | | |
| محمد قاري | Gari, N/A | Researcher | | |
| غازي دمنهوري | Damanbouri, N/A | Researcher | | |
| محمد فضل الله | Fadlallah, N/A | Researcher | | |
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